October 21, 2017
This is the academic website of Todd Wylie, bioinformatician and genomicist. I am an Assistant Professor at Washington University School of Medicine (St. Louis, MO). I develop/utilize bioinformatics methods and tools for the analysis of high throughput multi-omics data. You can find information about my research, publications, and scientific interests here.

Biography

HEADSHOTI have over 20 years experience in large-scale genomics, applying biology, informatics, and analytic skills to a wide range of scientific research. I am highly experienced in the technology development and informatics operations of a large genome center. Early in my career, I contributed to the International Human Genome Sequencing Consortium that sequenced and mapped the first human genome [PMID: 11237011]. Subsequently, I contributed to the sequencing and analysis of other landmark model organisms, including mouse, macaque, platypus, soybean, zebrafish, toxoplasma, and numerous parasitic and free-living nematode species. I contributed to the sequencing and publication of the first human cancer genome [PMID: 18987736], a patient with acute myeloid leukemia, as well as subsequent projects involving sequence data from human cancers. I worked 9 years in the area of technology development at The McDonnell Genome Institute (MGI) under Dr. Elaine R. Mardis, managing a small, agile group of bioinformaticians. This role required being the informatics/analysis "glue" between the development wet lab, bioinformatics team, testing and training, and other collaborative groups at the institute. As such, I have extensive experience handling and analyzing high throughput sequencing data as produced by multiple next-generation sequencing platforms. Prior to joining the Department of Pediatrics, I directed Dr. George Weinstock’s microbial computing group, focusing on software development and analysis for the study of human and microbial genomes. I also have an extensive background in targeted sequence capture, transcriptomics, and the analysis of whole exome data. My colleagues and I recently developed a comprehensive targeted sequence capture panel called ViroCap [PMID: 26395152], designed to enrich nucleic acid from DNA and RNA viruses. This tool will greatly enhance the study of eukaryotic DNA and RNA viruses and takes us closer to using high-throughput sequencing as a comprehensive viral diagnostic tool.

Mission Statement

As Department of Pediatrics and MGI faculty, my focus is to collaborate with clinicians and basic scientists in improving medical practice through the application of high-throughput multi-omics and associated computational methods. I am also currently involved in many collaborative efforts studying the microbiome and its association with disease.

Research Interests

genomics • human microbiome • bacterial and viral characterization • transcriptomics and RNA-seq • Next-generation sequencing (NGS) technologies and related analyses • applied bioinformatics solutions in large-scale research • R&D pipeline and analytical software development • exome and custom targeted sequencing platforms • early-access technology evaluation and application • data visualization, representation, and set theory approaches for big data

Recent Publications

Wylie KM, Blanco-Guzman M, Wylie TN, Lawrence SJ, Ghobadi A, DiPersio JF, Storch GA. High-throughput sequencing of cerebrospinal fluid for diagnosis of chronic Propionibacterium acnes meningitis in an allogeneic stem cell transplant recipient. Transpl Infect Dis. 2016 Apr;18(2):227-33. doi: 10.1111/tid.12512. Epub 2016 Mar 31. PubMed PMID: 26895706.
Rowley AH, Wylie KM, Kim KY, Pinku AJ, Yang A, Reindel R, Baker SC, Shulman ST, Orenstein JM, Lingen MW, Weinstock GM, Wylie TN. The transcriptional profile of coronary arteritis in Kawasaki disease. BMC Genomics. 2015 Dec 18;16:1076. doi: 10.1186/s12864-015-2323-5. PubMed PMID: 26679344; PubMed Central PMCID: PMC4683744.
Solga AC, Pong WW, Kim KY, Cimino PJ, Toonen JA, Walker J, Wylie T, Magrini V, Griffith M, Griffith OL, Ly A, Ellisman MH, Mardis ER, Gutmann DH. RNA Sequencing of Tumor-Associated Microglia Reveals Ccl5 as a Stromal Chemokine Critical for Neurofibromatosis-1 Glioma Growth. Neoplasia. 2015 Oct;17(10):776-88. doi: 10.1016/j.neo.2015.10.002. PubMed PMID: 26585233; PubMed Central PMCID: PMC4656811.
Wylie TN, Wylie KM, Herter BN, Storch GA. Enhanced virome sequencing using targeted sequence capture. Genome Res. 2015 Dec;25(12):1910-20. doi: 10.1101/gr.191049.115. Epub 2015 Sep 22. PubMed PMID: 26395152; PubMed Central PMCID: PMC4665012.
All Publications

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